The migration of the primordial germ cells ( PGCs), formation of the genital ridge, gonad sex differentiation, and their variations under different water temperatures were investigated using histological methods in the teleost, Pseudobagrus vachelli. 通过组织学方法观察江黄颡鱼原始生殖细胞(PGCs)迁移、生殖嵴生成和性腺分化,并且探讨在不同温度培育下性腺分化的差异。
The migratory path of PGCs is: endoderm of yolk sac → endoderm of intestinal area → dorsal mesentery → genital ridge. 原始生殖细胞迁移路径为:卵黄囊内胚层→肠区内胚层→背肠系膜→生殖嵴;
CONCLUSION: Time and methods of separating genital ridge and PGC clone are of primary importance in the culture of PGC. 结论:离散生殖嵴和增殖的PGC克隆的时机和方法是影响建系的关键;
Migratory mode: from endoderm of yolk sac to endoderm of intestinal area is passive, from dorsal mesentery to genital ridge is active. 迁移方式:从卵黄囊内胚层到肠区内胚层为被动迁移过程;背肠系膜到生殖嵴为主动迁移过程;
Isolation and culture of primordial germ cells from genital ridge in chicken embryos 鸡胚生殖嵴中原始生殖细胞的分离培养
Chicken embryonic stem cells were pluripotent stem cell which derived from stage blastoderm cells or PGCs isolated from genital ridge by long-time restraining differentiation in vitro. 鸡胚胎干细胞是一种多能性干细胞,从Ⅹ期胚盘分离胚盘细胞或早期鸡胚的生殖嵴分离原始生殖细胞,经体外长期抑制分化培养可得到鸡胚胎干细胞。